Reverse transcription was performed using a reverse-transcription system (Promega) according to the manufacturer’s protocols. Quantitative RT-PCR was performed using KAPA SYBR Fast qPCR kit (TAKARA, Code: DRR036A) and signals were detected with ABI 7500 Real-Time PCR System (Applied BioSystems). GAPDH was used as endogenous control. The RT-PCR was carried out in triplicate. All the primers used are listed in S1 Table .
Realtime quantification of microRNAs was carried out by stem-loop RT-PCR as described before [22 (link)]. Briefly, reverse transcription was performed using a reverse-transcription kit (TAKARA, code: DRRO47A) according to the manufacturer’s protocols. Quantitative RT-PCR was performed using KAPA SYBR Fast qPCR kit (TAKARA, Code: DRR420A) and signals were detected with ABI 7500 Real-Time PCR System (Applied BioSystems). RnU6 was used as endogenous control. The RT-PCR was carried out in triplicate. All the primers used are listed inS2 Table .
Realtime quantification of microRNAs was carried out by stem-loop RT-PCR as described before [22 (link)]. Briefly, reverse transcription was performed using a reverse-transcription kit (TAKARA, code: DRRO47A) according to the manufacturer’s protocols. Quantitative RT-PCR was performed using KAPA SYBR Fast qPCR kit (TAKARA, Code: DRR420A) and signals were detected with ABI 7500 Real-Time PCR System (Applied BioSystems). RnU6 was used as endogenous control. The RT-PCR was carried out in triplicate. All the primers used are listed in
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