Antibody Preparation and Characterization

Rabbit anti-JEV serum that exhibited cross-reactivity with the WNV E protein was prepared as described previously^{32 (link)–34 (link)}. The following antibodies were used: mouse anti-WNV E protein monoclonal antibody (Merck Millipore, Billerica, MA, USA), mouse anti-actin monoclonal antibody (Wako), rabbit anti-CD3E monoclonal antibody (Sigma-Aldrich, St. Louis, MO, USA), and rabbit anti-CD8a monoclonal antibody (Cell Signaling Technology, Beverly, MA, USA).

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Kobayashi S., Kaneko C., Kawakami R., Hasebe R., Sawa H., Yoshii K, & Kariwa H. (2020). Amino acid 159 of the envelope protein affects viral replication and T-cell infiltration by West Nile virus in intracranial infection. Scientific Reports, 10, 7168.

Publication 2020

mouse anti-WNV E protein monoclonal antibody mouse anti-actin monoclonal antibody

Actin Anti antibody Antibodies Cross reactivity Monoclonal antibody Mouse Protein Protein monoclonal Rabbit Serum

Corresponding Organization : Hokkaido University

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Variable analysis

independent variables

Rabbit anti-JEV serum that exhibited cross-reactivity with the WNV E protein

dependent variables

Cross-reactivity of the rabbit anti-JEV serum with the WNV E protein

control variables

Mouse anti-WNV E protein monoclonal antibody
Mouse anti-actin monoclonal antibody
Rabbit anti-CD3E monoclonal antibody
Rabbit anti-CD8a monoclonal antibody

controls

Positive control: Mouse anti-WNV E protein monoclonal antibody
Negative controls: Mouse anti-actin monoclonal antibody, Rabbit anti-CD3E monoclonal antibody, Rabbit anti-CD8a monoclonal antibody

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