Nucleotide sequences of OmpA SS and MP-V1 were collected via the National Center for Biotechnology Information (NCBI) and a previous study [25 (link)], respectively. The collected OmpA SS nucleotide sequence was designed to connect directly to the translational start site derived from the Ptrc promoter, and the MP-V1 was also designed to directly connect OmpA SS. The OmpA SS was fused directly to the Ptrc promoter without MP-V1, and was designed to be used as a negative control. The designed artificial genes were prepared by an artificial-gene synthesis (Bioneer Corp., Daejon, Korea) and then cloned into T-vector (Promega, Madison, WI, USA), finally resulting in the pMMP319 and pMMP320 (Figure 4 and Table 1 ).
General DNA manipulations were conducted as described by Sambrook et al. [44 ]. Plasmids were introduced into Top10 (Invitrogen, Carlsbad, CA, USA), E. coli competent cells, by heat-shock with RbCl2 treatment. Nucleotide sequencing was conducted by using an ABI 3730XI automatic sequencer (Applied Biosystems, Foster City, CA, USA). The E. coli strain and plasmids used for this study are listed in theTable 1 .
General DNA manipulations were conducted as described by Sambrook et al. [44 ]. Plasmids were introduced into Top10 (Invitrogen, Carlsbad, CA, USA), E. coli competent cells, by heat-shock with RbCl2 treatment. Nucleotide sequencing was conducted by using an ABI 3730XI automatic sequencer (Applied Biosystems, Foster City, CA, USA). The E. coli strain and plasmids used for this study are listed in the
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