Stress Response of Rice Seedlings to Abiotic Factors

Two-week-old seedlings of Shen Nong 9816 (SN9816) grown in 14 h light and 10 h dark were collected. The harvested seedlings were treated with 0 and 250 mM NaCl; 0 and 100 μM ABA; 0 and 100 μM JA for 6 h. Two biological replications were performed for each treatment. Total RNA of the samples was isolated using Takara RNAiso Plus (Takara Bio Inc., Otsu, Japan). The contimanate DNA was removed by using RQ1 RNase-Free DNase treatment (Promega, Madison, WI, USA). Then, 3 μg of treated RNA was used to synthesize the first-strand cDNA with the RevertAid First Strand cDNA Synthesis Kit (Fermentas, Waltham, MA, USA). SYBR Premix Ex Taq (Takara Bio Inc., Shiga, Japan) and a Q5 Real-Time PCR Instrument (Applied Biosystems, Waltham, MA, USA) were used for qRT-PCR [95 (link),96 (link)]. Primers used for qRT-PCR are listed (Table S2).
The Mircroarray data are from the Rice Expression Profile Database (RiceXPro, http://ricexpro.dna.affrc.go.jp/, accessed on 13 May 2021). Four-leaf-old seedlings of rice were treated with 50 μM ABA and 100 μM MeJA for 0, 1, 3, 6, and 12 h; two biological replicates were performed. The shoot of the treated seedlings was used for microarray analysis. The signal intensity detected during hybridization was used for drawing heatmap with TBtools.