Aortic Valve Atherosclerosis Imaging

Serial sections of the aortic valve were stained with oil red O and en face of the proximal aorta was stained with Sudan IV as previously reported (Luo et al., 2015b (link)). Briefly, obtained hearts were embedded with OCT, and serial 8-μm-thick frozen sections with 40-μm intervals were obtained and stained with oil red O. The images of plaque size and lesion area were analyzed using IPP 6.0 software (Media Cybernetics, Rockville, MD, United States).

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Qin M., Luo Y., Lu S., Sun J., Yang K., Sun G, & Sun X. (2017). Ginsenoside F1 Ameliorates Endothelial Cell Inflammatory Injury and Prevents Atherosclerosis in Mice through A20-Mediated Suppression of NF-kB Signaling. Frontiers in Pharmacology, 8, 953.

Publication 2017

IPP 6.0 software

Aorta Aortic valve Face Frozen sections Hearts Plaque Sudan iv

Corresponding Organization : State Administration of Traditional Chinese Medicine of the People's Republic of China

Other organizations : Zhejiang University of Technology

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Variable analysis

independent variables

Staining method (oil red O for serial sections of the aortic valve, Sudan IV for en face of the proximal aorta)

dependent variables

Plaque size
Lesion area

control variables

Thickness of frozen sections (8-μm-thick)
Interval between serial sections (40-μm)

controls

Positive control: Not specified
Negative control: Not specified

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