Culturing 16HBE14o- and Primary HBE Cells

The 16HBE14o- cell line was maintained in minimum essential medium (MEM) supplemented with 10 % fetal bovine serum, 1 % penicillin/streptomycin, and 1 % glutamine (Invitrogen, Carlsbad, CA) and cultured as described previously [47 (link)]. In some experiments, MEM with no phenol red (Invitrogen) was applied. Primary HBE cells were obtained from ScienCell Research Laboratories (Carlsbad, USA) and cultured using Bronchial Epithelial Cell Medium (ScienCell Research Laboratories) following the commercial protocol described previously [20 (link)].

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Hao Y., Chow A.W., Yip W.C., Li C.H., Wan T.F., Tong B.C., Cheung K.H., Chan W.Y., Chen Y., Cheng C.H, & Ko W.H. (2016). G protein-coupled estrogen receptor inhibits the P2Y receptor-mediated Ca2+ signaling pathway in human airway epithelia. Pflugers Archiv, 468, 1489-1503.

Publication 2016

MEM fetal bovine serum penicillin/streptomycin glutamine phenol red Bronchial Epithelial Cell Medium

Bronchial Cell line Cells Epithelial cell Fetal bovine serum Glutamine Penicillin Streptomycin

Corresponding Organization :

Other organizations : Chinese University of Hong Kong, University of Hong Kong

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Variable analysis

independent variables

Culture medium (MEM with or without phenol red)

dependent variables

Not explicitly mentioned

control variables

Cell line (16HBE14o-)
Cell culture conditions (10% fetal bovine serum, 1% penicillin/streptomycin, 1% glutamine)
Primary HBE cell culture conditions (Bronchial Epithelial Cell Medium)

controls

Positive control: Not specified
Negative control: Not specified

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