Quantifying Tissue Glucose Uptake in Mice

Upon completing the cognitive tests, three mice from each group were injected with D-[1-¹⁴C] glucose (cat: NEC043X001 MC, PerkinElmer, United States) via the tail vein. Briefly, mice were anesthetized with isoflurane and injected intravenously via the tail with 50 μCi of tracer diluted to a final volume of 20 μL in isotonic saline. Following a 15 min uptake period, the animals were killed, and tissues were collected. Tissue radioactivity was quantified by liquid scintillation. D-[1-¹⁴C] glucose levels were normalized to the weight of resected tissue and expressed as the percent of injected dose (Tsytsarev et al., 2012 (link); Cox et al., 2015 (link)).

Free full text: Click here

Cisternas P., Oliva C.A., Torres V.I., Barrera D.P, & Inestrosa N.C. (2019). Presymptomatic Treatment With Andrographolide Improves Brain Metabolic Markers and Cognitive Behavior in a Model of Early-Onset Alzheimer’s Disease. Frontiers in Cellular Neuroscience, 13, 295.

Publication 2019

D-[1-14C] glucose

Animals Cognitive tests Glucose Isoflurane Mice Radioactivity Saline Tail Tissue Vein

Corresponding Organization : UNSW Sydney

Top 5 similar protocols

Variable analysis

independent variables

Administration of D-[1-14C] glucose

dependent variables

Tissue radioactivity
D-[1-14C] glucose levels (normalized to tissue weight and expressed as percent of injected dose)

control variables

Anesthesia with isoflurane
Intravenous injection of tracer via tail vein
Uptake period of 15 minutes
Tissue collection after animals were killed

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!