Quantifying CILK1/ICK and KIF3A Interactions

Alvocidib/flavopiridol (S2679) and AT7519 (S1524) were from Selleckchem (Houston, TX, USA). Glutathione Sepharose 4B (17-0756-01) and Gammabind Plus Sepharose beads (17-0886-01) were from GE Healthcare (Chicago, IL, USA). Anti-FLAG tag affinity beads (ab270704) were from Abcam (Cambridge, MA, USA). pEBG-GST-CILK1/ICK encoding GST-CILK1/ICK was described in [13 (link),21 (link)]. pCMV6-Myc-Flag-CILK1 encoding CILK1-Myc-Flag (RC213609) was from Origene (Rockville, MD, USA). pCIG-HA-KIF3A encoding HA-KIF3A was described in [35 (link)].

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Wang E.X., Turner J.S., Brautigan D.L, & Fu Z. (2022). Modulation of Primary Cilia by Alvocidib Inhibition of CILK1. International Journal of Molecular Sciences, 23(15), 8121.

Publication 2022

AT7519 Glutathione Sepharose 4B Gammabind Plus Sepharose beads

At7519 Cilk1 Flavopiridol Glutathione Sepharose Sepharose 4b

Corresponding Organization : University of Virginia

Top 5 similar protocols

Variable analysis

independent variables

Alvocidib/flavopiridol (S2679)
AT7519 (S1524)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: pEBG-GST-CILK1/ICK encoding GST-CILK1/ICK
Positive control: pCMV6-Myc-Flag-CILK1 encoding CILK1-Myc-Flag (RC213609)
Positive control: pCIG-HA-KIF3A encoding HA-KIF3A

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