Dual IHC (ER/Ki67 and Ki67/PR) Staining Protocol

The dual IHC (ER/Ki67 and Ki67/PR) was performed by the Pathology Solid Tumor Core at City of Hope using Ventana Discovery Ultra IHC Auto Stainer (Roche Diagnostics). Primary antibodies used for the immunostaining include human ERα rabbit monoclonal antibody (Roche Diagnostics, 790−4325), human PR rabbit monoclonal antibody (Roche Diagnostics, 790−4296) and human Ki67 rabbit monoclonal antibody (Roche Diagnostics, 790−4286). Images were captured with VENTANA iScan HT (Roche Diagnostics) and analysed using the Cell Detection and Cell Classification functions implemented in Qupath software.
²⁴ (link) Staining protocol details are described in Supplementary Methods.

Free full text: Click here

Yoshitake R., Mori H., Ha D., Wu X., Wang J., Wang X., Saeki K., Chang G., Shim H.J., Chan Y, & Chen S. (2024). Molecular features of luminal breast cancer defined through spatial and single‐cell transcriptomics. Clinical and Translational Medicine, 14(1), e1548.

Publication 2024

Ventana Discovery Ultra IHC Auto Stainer VENTANA iScan HT

Corresponding Organization : City of Hope

Other organizations : Kyushu University, Okayama University of Science

Top 5 similar protocols

Variable analysis

independent variables

Ventana Discovery Ultra IHC Auto Stainer (Roche Diagnostics)

dependent variables

Immunostaining results for human ERα, human PR, and human Ki67

control variables

Tissue samples
Primary antibodies (human ERα rabbit monoclonal antibody, human PR rabbit monoclonal antibody, and human Ki67 rabbit monoclonal antibody)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!