RNA isolation and real-time PCR were performed according to protocols previously described in detail 16 (link). In brief, total mRNA from kidney tissue was extracted using the TRIzol reagent (RNA STAT 60 Tel-Test; Ambion, Austin, TX, USA). cDNA was used in the specific assays by Real-Time PCR system (Life Technologies Corporation, Carlsbad, CA, USA) on demand target mixes (Applied Biosystems, Foster City, CA, USA). The expression levels of mRNA encoding for TGF-β, FN, collagen I, and collagen IV in the kidney tissue were determined and normalized to the level of GAPDH (Applied Biosystems). The cycle times (Ct) were then compared to determine the fold differences between samples.
Protocol detail
Quantifying Kidney Gene Expression
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : First Hospital of Jilin University, Jilin University, University of Louisville, Union Hospital, Wenzhou Medical University, Ruian People's Hospital
Top 5 similar protocols
Variable analysis
independent variables
- None explicitly mentioned
- MRNA expression levels of TGF-β, FN, collagen I, and collagen IV in the kidney tissue
- GAPDH mRNA expression level (used for normalization)
- Positive control: None mentioned
- Negative control: None mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!