Genetic Regulation of Necroptosis and Oxidative Stress

All animal procedures were approved by the Animal Care and Use Committee of Zhejiang University. The generation of Ripk3^−/−, Mlkl^−/−, Fadd^−/−Mlkl^−/−, and Nrf2^−/− mice (17 (link), 49 (link), 50 (link)), LC/MS analysis, iron assay, and methods used in surgery, drug treatment, cell culture, histology, lipid peroxidation detection, and mitochondria isolation are provided in SI Appendix, SI Materials and Methods. Except where indicated otherwise, all summary data are presented as the mean ± SEM. The Student’s t test was used to compare two groups, and the log-rank test was used to analyze the survival curves. Differences with a P value of <0.05 were considered statistically significant.

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Fang X., Wang H., Han D., Xie E., Yang X., Wei J., Gu S., Gao F., Zhu N., Yin X., Cheng Q., Zhang P., Dai W., Chen J., Yang F., Yang H.T., Linkermann A., Gu W., Min J, & Wang F. (2019). Ferroptosis as a target for protection against cardiomyopathy. Proceedings of the National Academy of Sciences of the United States of America, 116(7), 2672-2680.

Publication 2019

Animal Assay Cell culture Drug Fadd Iron Isolation Lipid peroxidation Mice Mitochondria Nrf2 Ripk3 Student Surgery

Corresponding Organization :

Other organizations : Zhejiang University, Zhengzhou University, Shanghai Institutes for Biological Sciences, Shanghai Jiao Tong University, Chinese Academy of Sciences, Second Affiliated Hospital of Zhejiang University, Institute of Biophysics, University of Chinese Academy of Sciences, University Hospital Carl Gustav Carus, TU Dresden, Columbia University, Cancer Genetics (United States)

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Variable analysis

independent variables

Genotype: Ripk3-/-, Mlkl-/-, Fadd-/- Mlkl-/-, Nrf2-/-

dependent variables

Outcomes measured by LC/MS analysis
Iron assay
Histology
Lipid peroxidation detection
Mitochondria isolation

control variables

All animal procedures were approved by the Animal Care and Use Committee of Zhejiang University

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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