Lateral Ventricle Microinfusion in Rodents

Animals anaesthetized with 5% chloral hydrate in normal saline and placed in a stereotaxic apparatus (RWD Life Science) were implanted bilaterally with a guide cannula with stylus aimed to the paired lateral ventricles following the coordinates below: anteroposterior, −0.5 mm; lateral, ±1.0 mm; dorsoventral, −2.5 mm. The cannulas were positioned in place with acrylic dental cement and secured with skull screws. A stylus was placed in the guide cannula to prevent clogging^{43 (link)}. Animals were allowed to recover from surgery for a week before experimental manipulations. For microinfusion, the stylus was removed from the guide cannula, and an infusion cannula connected to a microsyringe via a tubing was inserted. Peptides (50 mM in artificial cerebrospinal fluid, 3 μl per side) were microinfused into lateral ventricles through the microsyringe driven by a microinfusion pump (KDS 310, KD Scientific) at the rate of 0.2 μl/min.

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Wang J.J., Liu F., Yang F., Wang Y.Z., Qi X., Li Y., Hu Q., Zhu M.X, & Xu T.L. (2020). Disruption of auto-inhibition underlies conformational signaling of ASIC1a to induce neuronal necroptosis. Nature Communications, 11, 475.

Publication 2020

KDS 310

Animals Cannula Cerebrospinal fluid Chloral hydrate Dental cement Lateral ventricles Normal saline Peptides Skull Surgery

Corresponding Organization :

Other organizations : Shanghai Center for Brain Science and Brain-Inspired Technology, Shanghai Jiao Tong University, National Health and Family Planning Commission, Zhejiang University, Chinese Academy of Medical Sciences & Peking Union Medical College, The University of Texas Health Science Center at Houston

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Variable analysis

independent variables

Microinfusion of peptides (50 mM in artificial cerebrospinal fluid, 3 μl per side)

dependent variables

Not explicitly mentioned

control variables

Animals were anaesthetized with 5% chloral hydrate in normal saline
Animals were placed in a stereotaxic apparatus (RWD Life Science)
Guide cannula with stylus aimed to the paired lateral ventricles following the coordinates: anteroposterior, −0.5 mm; lateral, ±1.0 mm; dorsoventral, −2.5 mm
The cannulas were positioned in place with acrylic dental cement and secured with skull screws
A stylus was placed in the guide cannula to prevent clogging
Animals were allowed to recover from surgery for a week before experimental manipulations

controls

Positive control: Not mentioned
Negative control: Not mentioned

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