1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) suspended in chloroform were obtained from Avanti Polar Lipids (Alabama, USA). Amphiphilic diblock copolymer, poly(butadiene-b-ethylene oxide) (PBD-b-PEO), catalog number P19015-BdEO, was acquired from Polymer Source, Inc. (Quebec, Canada). The polydispersity is 1.06 and its average molecular weight (Mn) is 4000 with PBD block (rich in 1,4 microstructure) 2500 and PEO block 1500, respectively. In this study, we chose PBD-b-PEO since the previous work showed formation of phase-separated hybrid vesicles with domains38 (link)–40 . Paclitaxel was purchased from Thermo Fisher Scientific (Waltham; MA, USA). DSM265 was purchased from MedChemExpress (MedChemExpress LLC; NJ, USA). All chemicals and materials were used as received.
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Hybrid Vesicle Formation with Lipids and Polymers
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Corresponding Organization : University of Illinois Urbana-Champaign
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Variable analysis
independent variables
- Concentrations of DOPC, DPPC, and PBD-b-PEO
- Presence or absence of paclitaxel and DSM265
- Formation of phase-separated hybrid vesicles with domains
- Solvents used (chloroform)
- Source of lipids and copolymer (Avanti Polar Lipids and Polymer Source, Inc.)
- Specific grades and properties of lipids and copolymer (DOPC, DPPC, PBD-b-PEO with Mn 4000, PBD 2500, PEO 1500, and polydispersity 1.06)
- Chemicals used (paclitaxel and DSM265)
- Positive control: Previous work showing formation of phase-separated hybrid vesicles with domains
- Negative control: Not explicitly mentioned
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