Total RNA extraction of the cells was extracted using TRIzol (Invitrogen) and 1.5 μg RNA was reverse transcribed by the First Strand cDNA Synthesis Kit (Marligen Biosciences). Quantitative real-time (qRT) PCR was performed by SYBR Green PCR Master Mix (Applied Biosystems) in the ABI 7300 Detection System (Applied Biosystems), using the primers related to all TRIM genes described in the Supplementary Table 1 20 (link),22 (link). The qRT-PCR primers related to sXBP1, ATF4, CHOP were showed in the Supplementary Table 1 39 (link).
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