Quantifying PD-L1 and ANLN in BLCA Tissues

In total, 21 BLCA tissue samples were acquired from patients who had undergone curative surgery at the Second Affiliated Hospital of Wenzhou Medical University. Ethical approval was obtained from the Second Affiliated Hospital of Wenzhou Medical University Research Ethics Committee. Antibodies against anillin (ANLN) (DF13590, 1 : 200; Affinity Biosciences), CD8 (PB9249, 1 : 200; BOSTER), and PD-L1 (66248-1-lg, 1 : 5000; Proteintech) were used for the immunohistochemical (IHC) staining of these proteins in the tissue samples, which was performed according to previously published methods [17 (link)]. For the IHC analysis, the H-score was applied to assess the expression levels of PD-L1 and ANLN [18 (link)]. The number of CD8+ cells was counted using the ImageJ software.

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Yang X., Zhou Y., Huang L., Lin S., Ye H, & Shan Y. (2022). Fibroblast Common Serum Response Signature-Related Classification Affects the Tumour Microenvironment and Predicts Prognosis in Bladder Cancer. Oxidative Medicine and Cellular Longevity, 2022, 5645944.

Publication 2022

PB9249

Anillin Antibodies Hospital ethics committee Patients Pd l1 Proteins Surgery Tissue

Corresponding Organization : Wenzhou Medical University

Other organizations : Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University

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Variable analysis

independent variables

Antibodies against anillin (ANLN)
Antibodies against CD8
Antibodies against PD-L1

dependent variables

Expression levels of PD-L1 and ANLN (measured using H-score)
Number of CD8+ cells (counted using ImageJ software)

control variables

21 BLCA tissue samples acquired from patients who had undergone curative surgery at the Second Affiliated Hospital of Wenzhou Medical University
Ethical approval obtained from the Second Affiliated Hospital of Wenzhou Medical University Research Ethics Committee

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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