Thalamus tissue powder was sonicated in 1M Tris (pH 7.5) membrane lysis buffer containing 1M NaCl, 1% Trition X-100, 5mM EDTA, proteinase inhibitor and phosphatase inhibitor. Supernatant was collected after 10 min centrifugation at 18,000 × g, 4°C. Protein concentration was measured by Bradford assay. The western blot procedure was the same as previously published (Pang et al. 2013 (link)). Briefly, proteins were separated on 4–20% criterion TGX gels (BioRad, 567–1094) and transferred overnight to Immobilon PVDF transfer membranes (Millipore, Billerica, MA, IPFL00010). Membranes were blocked over night at 4°C with primary antibodies and blocked 1 hr at room temperature with secondary antibodies. Primary antibodies purchased from Abcam (Cambridge, MA) included rabbit anti-GFAP (ab7260, 1:50,000), rabbit anti-GGT7 (ab80903, 1:1000), mouse anti-MAP2 (ab11267, 1:4000), mouse anti-MRP1 (ab32574, 1:500), mouse anti-CD68 (ab31630, 1:500). Mouse anti-NeuN was from Millipore (Billerica, MA, MAB377, 1:1000) and mouse anti-β-actin was from Santa Cruz (Santa Cruz, CA, sc-8432, 1:1000). Rabbit anti-GPx4 antibody was purchased from Cayman (Ann Arbor, MI, 10005258, 1:300) and goat anti-GPx1 was from R&D system (Minneapolis, MN, AF3798, 1:300). Secondary antibodies were from Li-Cor (Lincoln, NE).