Chitosan (CAS 9012-76-4; high MW: 310,000–375,000 Da) was purchased from Hangzhou Simit Chem. & Tech. Co. (Hangzhou, China). NeutraseTM 0.8 L enzyme was supplied by Novozymes A/S (Bagsværd, Denmark). Chitosan oligomers (COS) with a molecular weight of < 2000 Da were prepared according to the procedure reported by Santos-Moriano et al. [64 (link)], with the modifications indicated in [65 (link)].
1-heptacosanol (CAS 2004-39-9, 98%), 4,4,6,8-tetramethyl-2-chromanone (AldrichCPR T313513), vitamin E (α-tocopherol, CAS 10191-41-0, analytical standard), 2,2-diphenyl-1-picrylhydrazyl (DPPH, CAS 1898-66-4), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox, CAS 53188-07-1), methanol (CAS 67-56-1, UHPLC, suitable for MS), TSA (tryptic soy agar, CAS 91079-40-2) and TSB (tryptic soy broth, CAS 8013-01-2) were acquired from Sigma-Aldrich Química (Madrid, Spain). PDA (potato dextrose agar) was supplied by Becton Dickinson (Bergen County, NJ, USA). All reagents were used as supplied, without further purification.
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