1-heptacosanol (CAS 2004-39-9, 98%), 4,4,6,8-tetramethyl-2-chromanone (AldrichCPR T313513), vitamin E (α-tocopherol, CAS 10191-41-0, analytical standard), 2,2-diphenyl-1-picrylhydrazyl (DPPH, CAS 1898-66-4), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox, CAS 53188-07-1), methanol (CAS 67-56-1, UHPLC, suitable for MS), TSA (tryptic soy agar, CAS 91079-40-2) and TSB (tryptic soy broth, CAS 8013-01-2) were acquired from Sigma-Aldrich Química (Madrid, Spain). PDA (potato dextrose agar) was supplied by Becton Dickinson (Bergen County, NJ, USA). All reagents were used as supplied, without further purification.
Chitosan Oligomers Preparation and Characterization
1-heptacosanol (CAS 2004-39-9, 98%), 4,4,6,8-tetramethyl-2-chromanone (AldrichCPR T313513), vitamin E (α-tocopherol, CAS 10191-41-0, analytical standard), 2,2-diphenyl-1-picrylhydrazyl (DPPH, CAS 1898-66-4), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox, CAS 53188-07-1), methanol (CAS 67-56-1, UHPLC, suitable for MS), TSA (tryptic soy agar, CAS 91079-40-2) and TSB (tryptic soy broth, CAS 8013-01-2) were acquired from Sigma-Aldrich Química (Madrid, Spain). PDA (potato dextrose agar) was supplied by Becton Dickinson (Bergen County, NJ, USA). All reagents were used as supplied, without further purification.
Corresponding Organization : Universidad de Zaragoza
Other organizations : Universidad de Valladolid, Hospital Universitario Río Hortega
Variable analysis
- Chitosan concentration
- Chitosan molecular weight
- Neutrase enzyme
- Chitosan oligomer (COS) molecular weight
- Antioxidant activity
- Antimicrobial activity
- Chitosan source (Hangzhou Simit Chem. & Tech. Co.)
- Neutrase enzyme source (Novozymes A/S)
- Purity of chemicals (1-heptacosanol, 4,4,6,8-tetramethyl-2-chromanone, vitamin E, DPPH, Trolox, methanol)
- Culture media (TSA, TSB, PDA)
- Vitamin E (α-tocopherol)
- Trolox
- Not explicitly mentioned
Annotations
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