Histological Analysis of TA Muscle Damage

Histological examination was carried out as described previously [25 (link)]. In brief, TA muscles were collected at various time points and preserved for one day in formaldehyde (4% in PBS). Before embedding in paraffin, the tissue was dehydrated as per standard protocol. After the paraffin solidified, the blocks were cut into 6µm thick sections using a microtome. Hematoxylin and eosin (H&E) staining was used after deparaffinization to determine overall morphology and the presence of necrotic fibers following damage. An AMG EVOS fl microscope was used to photograph the sections (Thermo Fisher Scientific, Waltham, MA, USA).