E. histolytica strain HM-1:IMSS trophozoites were maintained in 50ml culture flasks (Greiner Bio-One) containing TYI-S-33 media, 10% heat-inactivated adult bovine serum (Sigma), 1% MEM Vitamin Solution (Gibco), supplemented with penicillin (100 U/mL) and streptomycin (100 μg/mL) (Omega Scientific) [14 (link)].
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