The SAMMY‐seq experiment was conducted as previously described (Marasca et al, 2016 (link); Sebestyen et al, 2020 (link)). In brief, 4 million AML12 cells were used for one sample. After 5 min treatment of 600 μl CSK buffer at 4°C, the supernatant was collected as S1. The pellets were washed and treated with 10 U Turbo DNase in 200 μl CSK buffer for 1 h at 37°C, the supernatant was collected as S2. Then, the pellets were washed and extracted with 2 M NaCl in CSK buffer for 10 min at 4°C. After centrifugation, the supernatant was collected as S3. The pellets were washed and solubilized in 8 M urea, and this fraction was collected as S4. For DNA sequencing, 50% volume of each fraction was extracted by phenol/chloroform and sonicated into 300–700 bp fragments. The DNA libraries were prepared using the VAHTS Universal DNA Library Prep Kit for Illumina V3 (Vazyme, ND607‐01) followed by next‐generation sequencing (NGS) using the Illumina HiSeq X Ten system. The remaining 50% of each fraction was lysed with SDS loading buffer and prepared for western blotting test.