Immunofluorescence assays (IFAs) were performed as previously described [52 (link)]. The primary antibodies used include rabbit/mouse anti-HA/Myc (Cell Signaling Technology), rabbit anti-IMC6 (1:1000), rabbit anti-Centrin1 (1:1000), rat anti-IMC3 (1:1000), and rabbit/mouse anti-acetylated-Tubulin (1:5000). A Nikon Eclipse E100080i microscope was used for imaging.
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Immunofluorescence Assay of Cellular Proteins
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Corresponding Organization : Indiana University – Purdue University Indianapolis
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- Primary antibodies used: rabbit/mouse anti-HA/Myc, rabbit anti-IMC6, rabbit anti-Centrin1, rat anti-IMC3, and rabbit/mouse anti-acetylated-Tubulin
- Immunofluorescence assay (IFA) results
- Microscope used: Nikon Eclipse E100080i
- Immunofluorescence assay (IFA) protocol: Performed as previously described [52 (link)]
- No positive or negative controls were explicitly mentioned in the provided information.
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