Hemolymph Analysis of Treated Larvae

The hemolymph samples were collected from treated larvae with concentrations of 50 and 100 μg/ml in the pre-pupal stage to determine the antioxidant and Catalase activity. The samples were collected by creating a slit within the proleg. Hemolymph that flowed from the wound without hand pressure was collected into Eppendorf tubes containing 0.025% of phenylthiourea as an anti-melanization. The mean number of circulating hemocytes per mm³ was calculated by the formula of Jones^{11 (link)}. To examine the differential hemocyte count (DHC), one hundred cells were identified to their typical hemocyte type after staining a smear of hemolymph with Wright's stain^{2 (link)}.

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Belal R, & Gad A. (2023). Zinc oxide nanoparticles induce oxidative stress, genotoxicity, and apoptosis in the hemocytes of Bombyx mori larvae. Scientific Reports, 13, 3520.

Publication 2023

Antioxidant Catalase Cells Hemocyte Hemolymph Larvae Phenylthiourea Pressure Pupal Wound

Corresponding Organization : Alexandria University

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Variable analysis

independent variables

Concentrations of 50 μg/ml
Concentrations of 100 μg/ml

dependent variables

Antioxidant activity
Catalase activity
Mean number of circulating hemocytes per mm^3
Differential hemocyte count (DHC)

control variables

Pre-pupal stage larvae
Hemolymph collected by creating a slit within the proleg
Hemolymph collected without hand pressure
Hemolymph collected into Eppendorf tubes containing 0.025% of phenylthiourea as an anti-melanization agent
Hemocytes identified after staining a smear of hemolymph with Wright's stain

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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