Celecoxib-loaded PCL Nanofiber Production

Celecoxib-loaded PCL nano fibers were produced by PCL (Mw = 80 kDa, Sigma-Aldrich, Saint Louis, USA) and Celecoxib (Sigma-Aldrich, Saint Louis, USA) according to a previously described method [12 (link)]. Firstly, 1 g PCL, 20 mg (0.2% wt/v), or 60 mg Celecoxib (0.6% wt/v) was added into 10 mL of 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP, Shanghai Darui Fine Chemical Co., Ltd) solution. Secondly, when the solution propulsion rate was set to 2 mL/h, the mixed solution was infused with a precision syringe pump. Meanwhile, the solution was electrospun with the voltage setting at 15.0 kV and collected by a slow-rotating mandrel placed 15 cm away from the needle tip. Lastly, the obtained Celecoxib-loaded PCL nano fibers were dried in a vacuum oven (LGJ-10C, Forging Technology Development (Beijing) Co., Ltd., China) at room temperature for 1 week to ensure complete removal of residual solvent.