HPAEC Analysis of Xylooligosaccharides

The XOS contents were evaluated by high-performance anion-exchange chromatography (HPAEC). Samples were dissolved in ultrapure water, oscillated by ultrasound, and then centrifuged at 6000 × g for 15 min, and 1 mL of the supernatant was collected. Standards of xylobiose (X2), xylotriose (X3), xylotetraose (X4), xylopentaose (X5), and xylohexaose (X6) were purchased from Megazyme (Wicklow, Ireland, UK). The following steps were conducted according to the method [18 (link)]. Analysis of the standards and filtered samples was carried out on a Dionex ICS3000 system equipped with a pump and an amperometric detector. The chameleon chromatography management system (Dionex, Sunnyvale, CA, USA) was used for sugar identification and quantification. An analytical CarboPac PA10 pellicular anion-exchange resin column (250 mm × 4 mm) was used for sugar separation. The monoses were eluted with 250 mmol/L NaOH at a flow rate of 1.0 mL/min.