LNCaP, 22RV-1 and VCaP cells were seeded in triplicate at a density of 5,000 cells/well into 96-well tissue culture plates, where they were allowed to adhere for 24 h and treated with/without 1 µM DOX and/or 30 µM Casodex® (Sigma-Aldrich; Merck KGaA) at 37°C for various indicated time periods. Relative cell viability was quantified by MTT analysis using a protocol described previously (34 (link)).
Protocol detail
Evaluating Cell Viability with Drugs
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Corresponding Organization :
Other organizations : The University of Texas Health Science Center at San Antonio, Xiangya Hospital Central South University, Central South University
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Variable analysis
independent variables
- Doxorubicin (DOX) at 1 μM
- Casodex® (Bicalutamide) at 30 μM
- Relative cell viability
- Cell density of 5,000 cells/well
- Incubation time of 24 h for cell adherence
- Incubation temperature of 37°C
- Treatment duration (various indicated time periods)
- Positive control: Cells treated with DOX and/or Casodex®
- Negative control: Cells without DOX and Casodex® treatment
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