Humanized Mouse Model of HIV Infection

Twenty weeks after humanization, 12 Hu-mice were infected intraperitoneally (IP) with 2 × 10⁴ tissue culture infectious dose 50 (TCID₅₀) of HIV-1_ADA (a macrophage tropic viral strain) (61 (link)–63 (link)). An additional six Hu-mice served as uninfected aged-matched controls. Peripheral blood samples were collected every four weeks via submandibular vein bleeding to assess HIV-1 viral RNA and to assess the dynamics of human immune cell markers by flow cytometric analysis. Plasma HIV-1 RNA levels were measured using an automated COBAS Ampliprep V2.0/Taqman-48 system (Roche Molecular Diagnostics, Basel, Switzerland) as per the manufacturer's instructions. The detection limit after dilution factor adjustment was 200 viral RNA copies/mL.

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Dash P.K., Alomar F.A., Cox J.L., McMillan J., Hackfort B.T., Makarov E., Morsey B., Fox H.S., Gendelman H.E., Gorantla S, & Bidasee K.R. (2021). A Link Between Methylglyoxal and Heart Failure During HIV-1 Infection. Frontiers in Cardiovascular Medicine, 8, 792180.

Publication 2021

COBAS Ampliprep V2.0/Taqman-48 system

Blood Cell Dilution Flow cytometric Hiv 1 Hiv infectious Human Macrophage Mice Molecular diagnostics Plasma Strain Tissue Vein Viral rna

Corresponding Organization : University of Nebraska Medical Center

Other organizations : Imam Abdulrahman Bin Faisal University

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Variable analysis

independent variables

Time after humanization (20 weeks)

dependent variables

HIV-1 viral RNA levels in peripheral blood
Dynamics of human immune cell markers in peripheral blood

control variables

Age of Hu-mice

controls

Positive control: 12 Hu-mice infected with 2 × 10^4 TCID50 of HIV-1ADA (a macrophage tropic viral strain)
Negative control: 6 uninfected aged-matched Hu-mice

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