Bone Mineral Content Analysis Protocol
Corresponding Organization : Yangzhou University
Variable analysis
- Removal of attachment on the right tibia
- Bone mineral content
- Drying the tibia at 105 °C for 48 h
- Defatting the tibia with ether for seven days and drying to constant weight at 105 °C
- Crushing the dried bones and putting them in the crucible
- Carbonizing the crushed bones on an electric furnace until smokeless
- Burning the crushed bones in a muffle furnace at 550–600 °C for 24 h
- Analyzing the tibia ash for calcium (Ca) using the Ethylene Diamine Tetraacetie Acid (EDTA) method according to the GB/T6436–2018
- Determining the phosphorus (P) in the tibia ash via the molybdate-yellow colorimetry method based on the GB/T6437–2018
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