Enzymatic degradation of the BPUR electrospun meshes was performed
in PBS containing 200 U/mL lipase (Sigma, from Thermomyces
lanuginosus) at 37 °C.43 (link) Electrospun specimens (40 mm
× 5 mm × 0.2 mm3) were placed into capped tubes
containing 2 mL of the lipase solution and incubated with shaking. Solutions
were changed twice per week. At each time point, specimens (n = 3) were
collected using centrifugation and carefully rinsed 3 times with distilled
water. Samples were subsequently frozen overnight and then lyophilized. The
percent mass loss of the degraded sample was determined after lyophilization
by dividing the mass loss by the initial dry mass.
in PBS containing 200 U/mL lipase (Sigma, from Thermomyces
lanuginosus) at 37 °C.43 (link) Electrospun specimens (40 mm
× 5 mm × 0.2 mm3) were placed into capped tubes
containing 2 mL of the lipase solution and incubated with shaking. Solutions
were changed twice per week. At each time point, specimens (n = 3) were
collected using centrifugation and carefully rinsed 3 times with distilled
water. Samples were subsequently frozen overnight and then lyophilized. The
percent mass loss of the degraded sample was determined after lyophilization
by dividing the mass loss by the initial dry mass.
