Immunoblotting of Exosomal Proteins

Western blotting was performed as described in a previous study (20 (link)). Antibodies targeting the following proteins were used: IκBα (CST4812, Cell Signaling Technology, Danvers, MA, USA), p65 (CST6956S, Cell Signaling Technology), CD9 (NBP2-22187, Novus Biologicals, Centennial, CO, USA), CD63 (EXOAB-CD63A-1, SBI, Tokyo, Japan), TSG101 (ab228013, Abcam, Cambridge, UK), GM130 (ab52649, Abcam), glyceraldehyde-3-phosphate dehydrogenase (GAPDH; sc-47724, Santa Cruz Biotechnology, Santa Cruz, CA, USA), histone H3 (ab1791, Abcam), mCherry (ab125096, Abcam), and GFP (CST2555, Cell Signaling Technology).

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Choi H., Kim Y., Mirzaaghasi A., Heo J., Kim Y.N., Shin J.H., Kim S., Kim N.H., Cho E.S., In Yook J., Yoo T.H., Song E., Kim P., Shin E.C., Chung K., Choi K, & Choi C. (2020). Exosome-based delivery of super-repressor IκBα relieves sepsis-associated organ damage and mortality. Science Advances, 6(15), eaaz6980.

Publication 2020

(EXOAB-CD63A-1, ab228013 ab52649 GAPDH; sc-47724 ab1791 ab125096

Antibodies Biologicals Gapdh Glyceraldehyde 3 phosphate dehydrogenase Histone h3 Iκbα Novus Proteins Tsg101

Corresponding Organization : Yonsei University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression levels of IκBα, p65, CD9, CD63, TSG101, GM130, GAPDH, histone H3, mCherry, and GFP

control variables

None explicitly mentioned

controls

No positive or negative controls explicitly mentioned

Annotations

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