Evaluating Anti-Inflammatory Compound CMC2.24

CMC2.24 compound with 99.5% purity [20 (link)] was kindly provided by Dr. Francis Johnson (Chem-Master Intl. Inc., Hauppauge, NY, USA). CMC2.24 was completely dissolved in dimethyl sulfoxide (DMSO) to a final concentration of 0.05 %. Medium plus 0.05 % DMSO was used as a solvent control. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Cat# ab37168), MMP-3 (Cat# ab52915), RUNX2 (Cat# ab23981), collagen 2a1 (Col2a1) (Cat# ab34712), Bcl-2 (Cat# ab196495), and vascular endothelial growth factor (VEGF) (Cat# ab214424) antibodies were obtained from Abcam (Cambridge, UK). Hif-2α (Cat# NB100-122) was obtained from Novus Biologicals (Littleton, Colorado, USA). Cleaved caspase-3 (Cat# 9664), phosphorylated-p65 (p-p65) (Cat# 3033), p65 (Cat# 8242), p-IκBα (Cat# 2859), and IκBα (Cat# 9242) antibodies were ordered from Cell Signaling Technology (Danvers, MA, USA). BMS-345541 (Cat# HY-10518) was obtained from MedChem Express (Monmouth Junction, NJ, USA). SNP (Cat# 1008) was procured from Youcare Pharmaceutical Group Co., Ltd (Beijing, China).

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Zhou Y., Ming J., Deng M., Li Y., Li B., Li J., Ma Y., Chen Z., Wang G, & Liu S. (2020). Chemically modified curcumin (CMC2.24) alleviates osteoarthritis progression by restoring cartilage homeostasis and inhibiting chondrocyte apoptosis via the NF-κB/HIF-2α axis. Journal of molecular medicine (Berlin, Germany), 98(10), 1479-1491.

Publication 2020

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) RUNX2 Bcl-2 Hif-2α Cleaved caspase-3 phosphorylated-p65 (p-p65) p-IκBα BMS-345541

Antibodies Bcl 2 Biologicals Bms 345541 Caspase 3 Cmc2 24 Collagen Compound 24 Dimethyl sulfoxide Glyceraldehyde 3 phosphate dehydrogenase Iκbα Mmp 3 Novus Pharmaceutical Runx2 Solvent Vascular endothelial growth factor

Corresponding Organization : SUNY Upstate Medical University

Other organizations : Wuhan University, Renmin Hospital of Wuhan University, Wuhan Union Hospital, Hubei University of Chinese Medicine

Top 5 similar protocols

Variable analysis

independent variables

CMC2.24 compound with 99.5% purity [20 (link)]

dependent variables

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
MMP-3
RUNX2
Collagen 2a1 (Col2a1)
Bcl-2
Vascular endothelial growth factor (VEGF)
Hif-2α
Cleaved caspase-3
Phosphorylated-p65 (p-p65)
P-IκBα
IκBα

control variables

Medium plus 0.05 % DMSO

positive controls

BMS-345541

Annotations

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