Genome-wide DNA methylation profiling

We extracted the genomic DNA (500 ng) from the saliva of the whole sample, however only 47 subjects paired by age, sex and different anxiety disorder trajectories due to logistical and financial limitations were included in the methylation analysis. The samples were treated with sodium bisulphite using the EZ-96 DNA Methylation-Gold Kit (Zymo Research, Orange, CA, USA) according to the manufacturer’s protocol. DNA methylation status was performed using the Infinium HumanMethylation450 (IHM450) BeadChip^{72 (link)} which covers 99% of Ref Seq genes regions and 96% of CpG islands/CpG island regions to explore the genome-wide DNA methylome^{72 (link)–74 (link)}. Data regarding pre-processing of raw data of IHM450 BeadChip and differential methylation analysis can be found elsewhere^{39 (link)}. All data of DNA methylation including methylated vs. unmethylated probes are deposited in GEO (GSE78975).

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Cerveira de Baumont A., Hoffmann M.S., Bortoluzzi A., Fries G.R., Lavandoski P., Grun L.K., Guimarães L.S., Guma F.T., Salum G.A., Barbé-Tuana F.M, & Manfro G.G. (2021). Telomere length and epigenetic age acceleration in adolescents with anxiety disorders. Scientific Reports, 11, 7716.

Publication 2021

EZ-96 DNA Methylation-Gold Kit

Anxiety disorder Cpg islands Dna methylation Genes Genome Gold Saliva Sodium bisulphite

Corresponding Organization : Universidade Federal do Rio Grande do Sul

Other organizations : The University of Texas Health Science Center at Houston, Pontifícia Universidade Católica do Rio Grande do Sul

Top 5 similar protocols

Variable analysis

independent variables

Anxiety disorder trajectories

dependent variables

Genome-wide DNA methylation status

control variables

Sodium bisulfite treatment using the EZ-96 DNA Methylation-Gold Kit (Zymo Research, Orange, CA, USA)
DNA methylation analysis using the Infinium HumanMethylation450 (IHM450) BeadChip

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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