siRNA Knockdown of Usp9X, Bag3, PMAIP1, Mcl-1

SignalSilence® Usp9X siRNA I #6308 was purchased from CST. Non-targeting siRNA-pool (ON-TARGETplus Non-targeting Pool, # D-001810-10-05) and siRNA against Bag3 (SMARTpool: ON-TARGETplus Bag3 siRNA, L-011957-00-0005), PMAIP1 siRNA and Mcl-1 (SMARTpool: ON-TARGETplus Mcl-1 siRNA, L-004501-00-0005) were purchased from Thermo Fisher Scientific (Pittsburgh, PA) and transfected as previously described [18 (link), 54 (link)]. Briefly, cells were incubated for 6 h with the formed complexes of Lipofectamine® 2000 (Invitrogen, Carlsbad, CA, U.S.A.) and the respective siRNA (12-well condition) in DMEM without FBS and antibiotics. After 6 h, FBS was added to a total concentration of 1.5%.