Dietary Fibers Attenuate DSS-Induced Colitis

All experimental animal procedures were approved by the Institutional Animal Care and Use Committee of Korea Institute of Oriental Medicine (KIOM-21-081) and performed in accordance with their guidelines. Six-week-old male C57/BL6 mice were purchased from DooYeol Biotech (Seoul, Republic of Korea) and divided into nine groups: vehicle-treated control (n = 10), 5% DSS (n = 10), 5% DSS + 2′-FL Low (n = 10), 5% DSS + 2′-FL High (n = 10), 5% DSS + 3-FL Low (n = 10), 5% DSS + 3-FL High (n = 10), 5% DSS + FOS Low (n = 10), 5% DSS + FOS High (n = 10), and 5% DSS + 100 mg/kg 5-ASA (n = 10). DSS-induced colitis mice were established by feeding mice 5% (wt/vol) DSS (MP Biomedicals, molecular weight of 36,000–50,000) dissolved in drinking water for 5 days, followed by 3 days of DSS-free water drinking [9 (link)]. Starting from the first day of DSS challenge, 2′-FL, 3-FL, FOS or 5-ASA was orally administered at the indicated dose per day, and weight was measured daily before administration. After the end of the experiment and sacrifice, colon length was measured and photographed.