Metabolic Profiling in Animal Model

The oxygen consumption (VO
₂), carbon dioxide production (VCO
₂), and respiratory exchange ratio (RER) were determined after 3 weeks of STM treatment using a Comprehensive Lab Animal Monitoring System (CLAMS)-Oxymax (Columbus Instruments, Columbus, USA). The energy expenditure was calculated using the formula VO
₂×[3.815+(1.232×RER)] and analysed using ANCOVA with body weight as a covariate via the MMPC.org ANCOVA data analysis tool, as previously described [
21 (link),
22 (link)] .

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Zhu Y., Li H., Ma M., Li D., Zhang O., Cai S., Wang Y., Chen D., Jin S., Ding C, & Xu L. (2022). Swertiamarin ameliorates diet-induced obesity by promoting adipose tissue browning and oxidative metabolism in preexisting obese mice: Swertiamarin reverses obesity by enhancing energy expenditure. Acta Biochimica et Biophysica Sinica, 55(1), 131-142.

Publication 2022

CLAMS)-Oxymax

Animal Body weight Carbon dioxide Energy expenditure Mmpc Oxygen consumption Respiratory

Corresponding Organization :

Other organizations : Wenzhou Medical University

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Variable analysis

independent variables

STM treatment

dependent variables

Oxygen consumption (VO2)
Carbon dioxide production (VCO2)
Respiratory exchange ratio (RER)
Energy expenditure

control variables

Body weight

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