Quantitative PCR Protocol for Gene Expression
Corresponding Organization :
Other organizations : Friedrich-Alexander-Universität Erlangen-Nürnberg, Universitätsklinikum Erlangen, University Hospital Regensburg, University Medical Center Freiburg, University of Freiburg, University of Münster
Variable analysis
- RNA isolation from cells and tissues
- Reverse transcription
- Quantitative real-time PCR
- Amplification of cDNA derived from β-actin for normalization
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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