Genetically Modified Mouse Models for Investigating LRP5/6 Signaling

Lrp5^-/- [42 (link)] and Lrp5^a214v(neo)/+ [21 (link)] mice were generated as described. Lrp5^fl/fl and Lrp6^fl/fl [22 (link)], Flk1^Breier-Cre [30 (link)], Rx-Cre [23 (link)] and CD11b-Cre [29 (link)] mice were kindly provided by Drs. Bart O. William, Kevin P. Campbell, Eric Swindell and Roland Baron, respectively. VE-Cad-Cre [24 (link)], Tie2-Cre [25 (link)], LysM-Cre [28 (link)] and tdTomato [27 (link)] mice were purchased from the Jackson Laboratory. Flk1^Breier-Cre, Rx-Cre, CD11b-Cre, VE-Cad-Cre and Tie2-Cre mice were all transgenic lines generated by fusing the Cre gene to a fragment of the promoter sequence of Flk1, Rx, CD11b, VE-Cad and Tie2, respectively. LysM-Cre mice were knock-ins, generated by targeted insertion of the Cre cDNA into the endogenous M lysozyme locus. When animals from different genetic backgrounds were crossed, littermate controls were used to avoid confounding effects.

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Huang W., Li Q., Amiry-Moghaddam M., Hokama M., Sardi S.H., Nagao M., Warman M.L, & Olsen B.R. (2016). Critical Endothelial Regulation by LRP5 during Retinal Vascular Development. PLoS ONE, 11(3), e0152833.

Publication 2016

VE-Cad-Cre Tie2-Cre LysM-Cre

Animals Cd11b Cdna Gene promoter Genetic backgrounds Lysozyme M locus Mice Tdtomato Transgenic

Corresponding Organization : Boston Children's Hospital

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Variable analysis

independent variables

Lrp5^-/-
Lrp5^a214v(neo)/+
Lrp5^fl/fl
Lrp6^fl/fl
Flk1^Breier-Cre
Rx-Cre
CD11b-Cre
VE-Cad-Cre
Tie2-Cre
LysM-Cre
TdTomato

dependent variables

Not explicitly mentioned

control variables

Littermate controls were used when animals from different genetic backgrounds were crossed

controls

Positive controls: None specified
Negative controls: None specified

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