Enzymatic Inhibition Profiling of Novel Compounds

To investigate the MAO-A and MAO-B enzyme inhibition profiles of the synthesized compounds, the fluorometric enzyme inhibition assay was carried out, as previously defined by us [30 (link),38 (link),39 (link),40 (link)]. Ampliflu™ Red (10-Acetyl-3,7-dihydroxyphenoxazine) was used as a fluorescence reagent in this assay. All reagents and enzymes (Ampliflu™ Red, peroxidase from horseradish, hMAO-A, hMAO-B, H2O2, tyramine hydrochloride, moclobemide and selegiline) were supplied by Sigma-Aldrich (Steinheim, Germany).

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Osmaniye D., Sağlık B.N., Acar Çevik U., Levent S., Kaya Çavuşoğlu B., Özkay Y., Kaplancıklı Z.A, & Turan G. (2019). Synthesis and AChE Inhibitory Activity of Novel Thiazolylhydrazone Derivatives. Molecules, 24(13), 2392.

Publication 2019

Ampliflu™ Red hMAO-A hMAO-B tyramine hydrochloride moclobemide selegiline

Assay Dihydroxyphenoxazine Enzyme assay Enzymes Fluorescence Fluorometric H2o2 Horseradish peroxidase Inhibition Mao a Mao b Moclobemide Selegiline Tyramine

Corresponding Organization : Eskişehir City Hospital

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Variable analysis

independent variables

Synthesized compounds

dependent variables

MAO-A enzyme inhibition profile
MAO-B enzyme inhibition profile

control variables

Ampliflu™ Red (10-Acetyl-3,7-dihydroxyphenoxazine) as a fluorescence reagent
Peroxidase from horseradish
HMAO-A enzyme
HMAO-B enzyme
Tyramine hydrochloride
Moclobemide
Selegiline

positive controls

Moclobemide (for MAO-A inhibition)
Selegiline (for MAO-B inhibition)

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