Affinity Purification of FLAG-Tagged Proteins
Corresponding Organization : Hokkaido University
Variable analysis
- Presence of ACR11-FLAG protein
- Proteins bound to anti-DYKDDDDK beads
- Proteins eluted from the beads
- Soluble proteins extracted from rosette leaves of wild-type plants grown for 4 weeks
- Incubation of soluble proteins with anti-DYKDDDDK beads for 15 min at 25 °C
- Washing the beads three times with ice-cold BN-solubilization buffer
- Elution of bound proteins with BN-solubilization buffer containing DYKDDDDK peptides or SDS extraction buffer
- Positive control: Soluble proteins from ACR11-FLAG plants
- Negative control: Soluble proteins from wild-type plants
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