Soluble proteins extracted from rosette leaves of wild-type and ACR11-FLAG plants grown for 4 weeks were incubated with anti-DYKDDDDK beads (Wako, Japan) for 15 min at 25 °C. After discarding the supernatants, the beads were washed three times with ice-cold BN-solubilization buffer10 (link). Bound proteins were eluted with the BN-solubilization buffer containing DYKDDDDK peptides (Wako) or with SDS extraction buffer.
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