Modulation of p38 and MRP1 in ARPE-19 Cells

ARPE-19 cells were transfected with p38 siRNA (90 pmol per 12-well) using lipofectamine 3000 (Invitrogen) according to instructions. The sequence of p38 siRNA was 5′- GCAUAAUGGCCGAGCUGUUTT -3′, which was referenced from the literature [67 (link)]. Both siRNA targeting p38 and scramble siRNA control were synthesized by GenePharma (Shanghai, China). After being transfected with siRNA for 24 h, cells were exposed to UCB (50 μM) or anisomycin (50 nM) for another 72 h. Then, Western blot was performed to detect the p38 and MRP1 expressions.

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Li P., Yang Y., Lin Z., Hong S., Jiang L., Zhou H., Yang L., Zhu L., Liu X, & Liu L. (2022). Bile Duct Ligation Impairs Function and Expression of Mrp1 at Rat Blood–Retinal Barrier via Bilirubin-Induced P38 MAPK Pathway Activations. International Journal of Molecular Sciences, 23(14), 7666.

Publication 2022

lipofectamine 3000 scramble siRNA control

Anisomycin Cells Lipofectamine Mrp1 Sirna Western blot

Corresponding Organization : China Pharmaceutical University

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Variable analysis

independent variables

P38 siRNA (90 pmol per 12-well)
UCB (50 μM)
Anisomycin (50 nM)

dependent variables

P38 expression
MRP1 expression

control variables

Lipofectamine 3000 (Invitrogen)
Scramble siRNA control

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