Liver Fatty Acid Profiling by GC-MS

The fatty acid profile of liver extracts was analysed by means of a ThermoFisher Scientific DSQII GC/MS system (TraceDSQII mass spectrometer, TraceGCUltra gaschromatograph), Xcalibur MS Software Version 2.1 (including NIST Mass Spectral Library, NIST 08), and Wiley Registry of Mass Spectral Data 8th Edition for the assignment of chemical structures to chromatographic peaks, as previously described [21 (link)]. Each identified peak was expressed as relative percentage areas of total methylated fatty acids (FAME). The reference standard was the Marine Oil FAME Mix from Restek (Superchrom S.r.l., Cernusco sul Naviglio, Milan, Italy). Dichloromethane was used as a blank to avoid carryover from the previous analysis.

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Di Pasqua L.G., Berardo C., Cagna M., Mannucci B., Milanesi G., Croce A.C., Ferrigno A, & Vairetti M. (2021). Long-term cold storage preservation does not affect fatty livers from rats fed with a methionine and choline deficient diet. Lipids in Health and Disease, 20, 78.

Publication 2021

DSQII GC

Chromatographic Dichloromethane Fatty acids Gaschromatograph Gc ms Library Liver extracts Marine

Corresponding Organization : University of Pavia

Other organizations : National Research Council

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Relative percentage areas of total methylated fatty acids (FAME)

control variables

ThermoFisher Scientific DSQII GC/MS system (TraceDSQII mass spectrometer, TraceGCUltra gaschromatograph)
Xcalibur MS Software Version 2.1 (including NIST Mass Spectral Library, NIST 08)
Wiley Registry of Mass Spectral Data 8th Edition
Marine Oil FAME Mix from Restek (Superchrom S.r.l., Cernusco sul Naviglio, Milan, Italy) as reference standard
Dichloromethane used as a blank to avoid carryover from the previous analysis

positive controls

Marine Oil FAME Mix from Restek (Superchrom S.r.l., Cernusco sul Naviglio, Milan, Italy) as reference standard

negative controls

Dichloromethane used as a blank to avoid carryover from the previous analysis

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