Volatile Profiling of Saffron and Freesia

In order to detect volatile components of saffron and freesia “f2” flowers, we carried out a gas chromatography-mass spectrometry (GC-MS) analysis. Each 0.1 g of their dried materials was ground with an addition of 0.3 ml of water (H₂O) and 0.3 ml of MeOH, subsequently extracted with 0.6 ml of chloroform (by mixing and centrifugation), and the chloroform extraction was repeated by adding 0.5 ml of H₂O, and finally, each chloroform phase was concentrated to threefold by decompression. The prepared samples were subjected to GC-MS using Shimadzu GCMS-QP5050 (Shimadzu, Kyoto, Japan), as described (15 (link)).