Protocol detail

Derivation and Characterization of OCI Cell Lines

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The derivation of OCI-C1p, OCI-C4p, OCI-C5x, and OCI-P5x cells was previously described (16 (link)). OCI-cells were cultured in OCMI-L, a specific growth medium developed for these cells (US Biological, Cat # 506390). The OCI cells are available from the Ince laboratory. The passage number of OCI cells at the time of each experiment was from 40 to 60. TOV21G cells were obtained from ATCC and cultured in 1:1 of Medium 199 (Gibco®) and MCDB105 (Cell Application Inc.) with 15% fetal bovine serum (FBS). RMG1 cells were obtained from JCRB cell bank and maintained in RPMI with 20% FBS. Dicer-substrate small interfering RNAs (dsiRNAs) targeting BRD2, BRD3, BRD4 as well as negative control dsiRNA were purchased from Integrated DNA Technologies. AllStars Hs Cell Death Control siRNA was purchased from QIAGEN. JQ1 (13 (link)), CP203 (13 (link), 17 (link)), CPI0610 (18 (link)), Alpelisib (BYL719) (19 (link)) and MK2206 (20 (link)) were purchased from Selleckchem.

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Shigeta S., Lui G.Y., Shaw R., Moser R., Gurley K.E., Durenberger G., Rosati R., Diaz R.L., Ince T.A., Swisher E.M., Grandori C, & Kemp C.J. (2021). Targeting BET proteins BRD2 and BRD3 in combination with PI3K-AKT inhibition as a therapeutic strategy for ovarian clear cell carcinoma. Molecular cancer therapeutics, 20(4), 691-703.

Publication 2021

Medium 199 AllStars Hs Cell Death Control siRNA CPI0610 Alpelisib (BYL719) MK2206

Alpelisib Biological Brd4 Byl719 Cell Cell death Dicer Fetal bovine serum Mk2206 Sirna

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Variable analysis

independent variables

BRD2 dsiRNA
BRD3 dsiRNA
BRD4 dsiRNA
Negative control dsiRNA
AllStars Hs Cell Death Control siRNA
CP203
CPI0610
Alpelisib (BYL719)
MK2206

dependent variables

Not explicitly mentioned

control variables

Cell lines (OCI-C1p, OCI-C4p, OCI-C5x, OCI-P5x, TOV21G, RMG1)
Cell culture media (OCMI-L, 1:1 Medium 199 and MCDB105 with 15% FBS, RPMI with 20% FBS)
Passage number of OCI cells (40 to 60)

positive controls

AllStars Hs Cell Death Control siRNA

negative controls

Negative control dsiRNA

Annotations

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