Modulation of BEAS-2B Cells by TGF-β1 and FGF2

BEAS-2B cells were incubated with TGFβ1 (2 ng/ml) alone, FGF2 (2 nM) alone, PD173074 (0.1 μM, Cayman Chemical, MI, USA) alone or FGF2 (2 nM) and TGFβ1 with or without PD173074 for 4 days prior to collection of RNA. The dose of PD173074 used was based on our prior studies (53 (link)).

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El-Baz L.M., Shoukry N.M., Hafez H.S., Guzy R.D, & Salem M.L. (2020). Fibroblast Growth Factor 2 Augments Transforming Growth Factor Beta 1 Induced Epithelial-mesenchymal Transition in Lung Cell Culture Model. Iranian journal of allergy, asthma, and immunology, 19(4), 348-361.

Publication 2020

PD173074

Cayman Cells Fgf2 Pd173074 Tgfβ1

Corresponding Organization :

Other organizations : Suez University, University of Chicago, Tanta University

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Variable analysis

independent variables

TGFβ1 (2 ng/ml)
FGF2 (2 nM)
PD173074 (0.1 μM, Cayman Chemical, MI, USA)

dependent variables

RNA collection

control variables

BEAS-2B cells

controls

Positive control: FGF2 (2 nM) and TGFβ1 with PD173074
Negative control: TGFβ1 (2 ng/ml) alone, FGF2 (2 nM) alone, PD173074 (0.1 μM) alone

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