Packed mitochondria, vacuoles,
and whole cells were diluted 2–4 fold with deionized water.
The resulting ca. 400 μL suspensions were transferred to screw-top
plastic tubes and heated at 95 °C overnight in 200 μL of
30% trace-metal grade HNO3 (Fisher Scientific). Fe concentrations
were measured by ICP-MS (Agilent 7700x) as described.26 (link) Reported Fe concentrations of mitochondria, vacuoles, and
whole cells were multiplied by dilution factors and adjusted using
previously reported packing efficiencies of 0.77 for mitochondria,
0.76 for vacuoles, and 0.70 for whole cells.13 (link),28 (link)
and whole cells were diluted 2–4 fold with deionized water.
The resulting ca. 400 μL suspensions were transferred to screw-top
plastic tubes and heated at 95 °C overnight in 200 μL of
30% trace-metal grade HNO3 (Fisher Scientific). Fe concentrations
were measured by ICP-MS (Agilent 7700x) as described.26 (link) Reported Fe concentrations of mitochondria, vacuoles, and
whole cells were multiplied by dilution factors and adjusted using
previously reported packing efficiencies of 0.77 for mitochondria,
0.76 for vacuoles, and 0.70 for whole cells.13 (link),28 (link)
