Acidovorax sp. A1169 Carbon Utilization

Carbon source utilization abilities of Acidovorax sp. A1169 were assessed using GEN III Microplates (Biolog Inc., Hayward, CA, USA) as described in Gawor et al. (2016 (link)). After incubation in R3A broth on a rotary shaker (WL-972, JWElectronics) for 3 days in 15 °C the cells were harvested by centrifugation (9000 rpm for 3 min), washed twice, suspended in sterile 0.9% saline and added to a vial of MicroPlate IF C inoculation fluid until transmittance reached 90%. Biolog GEN III Microplates (Biolog Inc., Hayward, CA, USA) were inoculated according to the manufacturer’s instructions. The plates were incubated in darkness at 15 °C, the color development was read at 590 nm (A590) in a Varioskan plate reader (Thermo Fisher Scientific, Waltham, MA, USA), and cellular respiration was measured kinetically by determining the colorimetric reduction of tetrazolium dye. Data were collected twice a week over a 15-day period.