Preparation of Polyphenol Standards

(+)-Catechin, (−)-epicatechin, (−)-gallocatechin-3-O-gallate, (−)-epigallocatechin-3-O-gallate, procyanidin B₁, procyanidin B₂, procyanidin
C₁, and procyanidin A₂ were obtained from Chromadex,
Inc. (Irvine, CA, USA). The standards were vacuum-dried using a vacuum
drying box (National Appliance Co., Portland, OR, USA) at 110 °C
until a constant weight was reached (about 24 h). These dried standards
were used to determine the MRRF that were used for calibration.^{25 (link)}

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Lin L.Z., Sun J., Chen P., Monagas M.J, & Harnly J.M. (2014). UHPLC-PDA-ESI/HRMSn Profiling Method To Identify and Quantify Oligomeric Proanthocyanidins in Plant Products. Journal of Agricultural and Food Chemistry, 62(39), 9387-9400.

Publication 2014

(+)-Catechin (−)-epicatechin procyanidin B1 procyanidin B2 procyanidinC1 procyanidin A2

Catechin Epicatechin Epigallocatechin 3 o gallate Gallocatechin 3 gallate Mrrf Procyanidin a2 Procyanidin b1 Procyanidin b2 Vacuum

Corresponding Organization :

Other organizations : Beltsville Human Nutrition Research Center, Agricultural Research Service, United States Pharmacopeial Convention

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Protocol cited in 2 other protocols

Variable analysis

independent variables

(+)-Catechin
(−)-epicatechin
(−)-gallocatechin-3-O-gallate
(−)-epigallocatechin-3-O-gallate
Procyanidin B1
Procyanidin B2
Procyanidin C1
Procyanidin A2

dependent variables

MRRF that were used for calibration

control variables

Vacuum-drying at 110°C until constant weight (about 24 hours)

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