ChIP-PCR and ChIP-seq experiments were performed as previously described (17 (link)). Antibodies for histone modifications (anti-H3K4me, anti-H3K27ac, anti-H3K27me3 and anti-H3K36me3) were obtained from Millipore (Billerica, MA, USA). An anti-Flag M2 antibody (Sigma-Aldrich Corporation, St. Louis, MO, USA) was used in the ChIP experiments involving Flag-BmHP1a.
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Chromatin immunoprecipitation protocol
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Corresponding Organization :
Other organizations : The University of Tokyo
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Variable analysis
independent variables
- Antibodies for histone modifications (anti-H3K4me, anti-H3K27ac, anti-H3K27me3 and anti-H3K36me3)
- Anti-Flag M2 antibody (used in the ChIP experiments involving Flag-BmHP1a)
- Outcomes measured in the ChIP-PCR and ChIP-seq experiments
- Experimental conditions and procedures described as previously published (17)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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