About 300 oocytes of each group were briefly washed in phosphate-buffered saline and then lysed in 2× SDS buffer and stored at −80°C until use. Immunoblot analysis was preformed as described (Huang et al., 2015 (link)). For immunolabeling, the following primary antibodies and dilutions were used: goat anti–SPAG-1 antibody (1:200), anti–phospho-AMPKα antibody (1:1500), anti–phospho-cyclin B1 antibody (1:1000), anti–histone H3 (acetyl K9) antibody (1:1000), rabbit anti–cyclin B2 polyclonal antibody (1:200; Santa Cruz Biotechnology), rabbit anti–α-tubulin monoclonal antibody (1:1000; Cell Signaling Technology), rabbit anti–acetylated-α-tubulin (1:1000; Cell Signaling Technology), anti–phospho-H2A.X antibody (1:1000; Cell Signaling Technology), rabbit anti–phospho-p44/42 MAPK antibody (1:1000), rabbit anti-PKC monoclonal antibody (1:1000; Abcam), anti-CDC42 antibody (1:1000; Abcam), anti–N-WSAP antibody (1:1000; Abcam), anti-Arp3 antibody (1:1000; Abcam), and anti-BubR1 antibody (1:1000). The data were normalized to β-actin.