Reverse Genetics for Rotavirus Strains

RV strains used were simian RV SA11 strain (G3P[2]) and bovine RV RF strain (G6P[1]) and were rescued using reverse genetics (detailed below) (42 (link), 71 (link), 95 (link), 96 (link)). The natural isolate of the RF strain was a kind gift from the laboratory of Dr. Ulrich Desselberger (University of Edinburgh, UK). Viruses were propagated in MA104 cells cultured in DMEM supplemented with 0.5 μg/mL porcine pancreatic trypsin type IX (Sigma-Aldrich), and viral titers were measured by plaque assay as described below.

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Diebold O., Gonzalez V., Venditti L., Sharp C., Blake R.A., Tan W.S., Stevens J., Caddy S., Digard P., Borodavka A, & Gaunt E. (2022). Using Species a Rotavirus Reverse Genetics to Engineer Chimeric Viruses Expressing SARS-CoV-2 Spike Epitopes. Journal of Virology, 96(14), e00488-22.

Publication 2022

porcine pancreatic trypsin type IX

Assay Bovine Cells Pancreatic Plaque Porcine Strain Trypsin Viruses

Corresponding Organization : University of Cambridge

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Variable analysis

independent variables

Simian RV SA11 strain (G3P[2])
Bovine RV RF strain (G6P[1])

dependent variables

Viral titers measured by plaque assay

control variables

MA104 cells cultured in DMEM supplemented with 0.5 μg/mL porcine pancreatic trypsin type IX (Sigma-Aldrich)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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