Immunostaining of Neurogenesis Markers

Immunodetection of proliferation, progenitor and neuronal markers, and BrdU was carried out as previously described^{7 (link),93 (link)}. Primary antibodies used were rabbit anti-DCX (1:750, Cell Signaling Technology Inc.), goat anti-DCX (1:250, Santa Cruz Biotechnology), rabbit anti-Ki67 (1:250, Abcam), mouse anti-NeuN (1:300, Millipore), mouse anti-GFAP (1:8000, Sigma), goat anti-Sox2 (1:2000, R&D Systems), rat anti-BrdU (1:300, Abcam). As secondary antibodies Alexa (1:500, Molecular Probes) and DyLight (1:500, Abcam) conjugated antibodies were used. NucBlue (Life Technologies) was used as nuclear dye. Slices were mounted on gelatin-coated slides with Fluoromont-G (Electron Microscopy Sciences).

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Arredondo S.B., Reyes D.T., Herrera-Soto A., Mardones M.D., Inestrosa N.C, & Varela-Nallar L. (2021). Andrographolide promotes hippocampal neurogenesis and spatial memory in the APPswe/PS1ΔE9 mouse model of Alzheimer’s disease. Scientific Reports, 11, 22904.

Publication 2021

rabbit anti-DCX goat anti-DCX rabbit anti-Ki67 mouse anti-NeuN mouse anti-GFAP goat anti-Sox2 rat anti-BrdU NucBlue Fluoromont-G

Antibodies Brdu Electron microscopy Gelatin Gfap Goat Molecular probes Mouse Neuronal Rabbit Sox2

Corresponding Organization : Pontificia Universidad Católica de Chile

Other organizations : Universidad Andrés Bello

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Variable analysis

independent variables

Primary antibodies used were rabbit anti-DCX, goat anti-DCX, rabbit anti-Ki67, mouse anti-NeuN, mouse anti-GFAP, goat anti-Sox2, rat anti-BrdU

dependent variables

Immunodetection of proliferation, progenitor and neuronal markers, and BrdU

control variables

NucBlue (Life Technologies) was used as nuclear dye
Slices were mounted on gelatin-coated slides with Fluoromont-G (Electron Microscopy Sciences)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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